In collaboration with the Kaye lab at Brigham and Women's Hospital, we are quantifying the dynamics of latent Kaposi's sarcoma-associated herpesvirus (KSHV) persistence. We developed a mathematical model and a statistical inference framework to infer viral dynamics from fluorescence microscopy images of cells in culture. Forward simulations were used to understand decades-long viral persistence and evaluate latent KSHV replication as a potential therapeutic target to disrupt KSHV-dependent tumor growth.